Active Recombinant Mouse NOG protein(Met1-Cys232), hFc-tagged

• Cat.No.: NOG-887M
• Product Overview: Recombinant Mouse NOG (P97466) (Met1-Cys232) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-hFc
• Protein length: Met1-Cys232
• Form: Lyophilized from sterile PBS, pH7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.1-0.6μg/mL.
• Molecular Mass: The recombinant mouse NOG/Fc comprises 454 amino acids and has a predicted molecular mass of 51 kDa. It migRates as approximately 60 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Nog noggin [ Mus musculus ]
• Official Symbol: NOG
• Synonyms: NOG; noggin
• Gene ID: 18121
• mRNA Refseq: NM_008711
• Protein Refseq: NP_032737