Recombinant Mouse Nudt16l1 Protein, Myc/DDK-tagged

• Cat.No.: Nudt16l1-4537M
• Product Overview: Purified recombinant protein of mouse full-length nudix (nucleoside diphosphate linked moiety X)-type motif 16-like 1 (cDNA clone MGC:35707 IMAGE:5322456),, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Key regulator of TP53BP1 required to stabilize TP53BP1 and regulate its recruitment to chromatin. In absence of DNA damage, interacts with the tandem Tudor-like domain of TP53BP1, masking the region that binds histone H4 dimethylated at 'Lys-20' (H4K20me2), thereby preventing TP53BP1 recruitment to chromatin and maintaining TP53BP1 localization to the nucleus. Following DNA damage, ATM-induced phosphorylation of TP53BP1 and subsequent recruitment of RIF1 leads to dissociate NUDT16L1/TIRR from TP53BP1, unmasking the tandem Tudor-like domain and allowing recruitment of TP53BP1 to DNA double strand breaks (DSBs). Binds U8 snoRNA.
• Molecular Mass: 16.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Nudt16l1 nudix (nucleoside diphosphate linked moiety X)-type motif 16-like 1 [ Mus musculus (house mouse) ]
• Official Symbol: Nudt16l1
• Synonyms: Nudt16l1; nudix (nucleoside diphosphate linked moiety X)-type motif 16-like 1; Sdos; 1110001K21Rik; 5330437I08Rik; tudor-interacting repair regulator protein; NUDT16-like protein 1; protein syndesmos
• Gene ID: 66911
• mRNA Refseq: NM_025839
• Protein Refseq: NP_080115
• UniProt ID: Q8VHN8