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Recombinant Mouse P4HB Protein, His-tagged

Cat.No.: P4HB-33M
Product Overview: Recombinant mouse P4HB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description: P4HB, also known as protein disulfide-isomerase, prolyl 4-hydroxylase subunit beta, protocollagen hydroxylase, cellular thyroid hormone binding protein p55 and glutathione-insulin transhydrogenase, is an abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell.
Source: Insect cell
Species: Mouse
Tag: His
Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Molecular Mass: 56.1kDa (498aa)50-70KDa (SDS–PAGE under reducing conditions)
AA Sequence: DALEEEDNVL VLKKSNFEEA LAAHKYLLVE FYAPWCGHCK ALAPEYAKAA AKLKAEGSEI RLAKVDATEE SDLAQQYGVR GYPTIKFFKNGDTASPKEYT AGREADDIVN WLKKRTGPAA TTLSDTAAAE SLVDSSEVTV IGFFKDVESD SAKQFLLAAE AIDDIPFGIT SNSGVFSKYQLDKDGVVLFK KFDEGRNNFE GEITKEKLLD FIKHNQLPLV IEFTEQTAPK IFGGEIKTHI LLFLPKSVSD YDGKLSSFKR AAEGFKGKILFIFIDSDHTD NQRILEFFGL KKEECPAVRL ITLEEEMTKY KPESDELTAE KITEFCHRFL EGKIKPHLMS QEVPEDWDKQ PVKVLVGANFEEVAFDEKKN VFVEFYAPWC GHCKQLAPIW DKLGETYKDH ENIIIAKMDS TANEVEAVKV HSFPTLKFFP ASADRTVIDY NGERTLDGFKKFLESGGQDG AGDDEDLDLE EALEPDMEED DDQKAVKDEL LEHHHHHH
Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity: >95% by SDS – PAGE
Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration: 0.5mg/ml (determined by Absorbance at 280nm)
Gene Name: P4hb prolyl 4-hydroxylase, beta polypeptide [ Mus musculus ]
Official Symbol: P4HB
Synonyms: P4HB; prolyl 4-hydroxylase, beta polypeptide; protein disulfide-isomerase; p55; ER protein 59; protein disulfide isomerase; prolyl 4-hydroxylase subunit beta; cellular thyroid hormone-binding protein; endoplasmic reticulum resident protein 59; PDI; Thbp; ERp59; Pdia1;
Gene ID: 18453
mRNA Refseq: NM_011032
Protein Refseq: NP_035162
MIM:
UniProt ID:
Pathway: Chylomicron-mediated lipid transport, organism-specific biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Lipoprotein metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem;
Function: electron carrier activity; isomerase activity; procollagen-proline 4-dioxygenase activity; protein disulfide isomerase activity; protein disulfide oxidoreductase activity; protein heterodimerization activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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