Recombinant Mouse P4HB Protein, His-tagged

• Cat.No.: P4HB-33M
• Product Overview: Recombinant mouse P4HB, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: P4HB, also known as protein disulfide-isomerase, prolyl 4-hydroxylase subunit beta, protocollagen hydroxylase, cellular thyroid hormone binding protein p55 and glutathione-insulin transhydrogenase, is an abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. At the cell surface, it seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell.
• Source: Insect cell
• Species: Mouse
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 56.1kDa (498aa)50-70KDa (SDS–PAGE under reducing conditions)
• AA Sequence: DALEEEDNVL VLKKSNFEEA LAAHKYLLVE FYAPWCGHCK ALAPEYAKAA AKLKAEGSEI RLAKVDATEE SDLAQQYGVR GYPTIKFFKNGDTASPKEYT AGREADDIVN WLKKRTGPAA TTLSDTAAAE SLVDSSEVTV IGFFKDVESD SAKQFLLAAE AIDDIPFGIT SNSGVFSKYQLDKDGVVLFK KFDEGRNNFE GEITKEKLLD FIKHNQLPLV IEFTEQTAPK IFGGEIKTHI LLFLPKSVSD YDGKLSSFKR AAEGFKGKILFIFIDSDHTD NQRILEFFGL KKEECPAVRL ITLEEEMTKY KPESDELTAE KITEFCHRFL EGKIKPHLMS QEVPEDWDKQ PVKVLVGANFEEVAFDEKKN VFVEFYAPWC GHCKQLAPIW DKLGETYKDH ENIIIAKMDS TANEVEAVKV HSFPTLKFFP ASADRTVIDY NGERTLDGFKKFLESGGQDG AGDDEDLDLE EALEPDMEED DDQKAVKDEL LEHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >95% by SDS – PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: P4hb prolyl 4-hydroxylase, beta polypeptide [ Mus musculus ]
• Official Symbol: P4HB
• Synonyms: P4HB; prolyl 4-hydroxylase, beta polypeptide; protein disulfide-isomerase; p55; ER protein 59; protein disulfide isomerase; prolyl 4-hydroxylase subunit beta; cellular thyroid hormone-binding protein; endoplasmic reticulum resident protein 59; PDI; Thbp; ERp59; Pdia1
• Gene ID: 18453
• mRNA Refseq: NM_011032
• Protein Refseq: NP_035162
• Pathway: Chylomicron-mediated lipid transport, organism-specific biosystem; Lipid digestion, mobilization, and transport, organism-specific biosystem; Lipoprotein metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of lipids and lipoproteins, organism-specific biosystem; Protein processing in endoplasmic reticulum, organism-specific biosystem; Protein processing in endoplasmic reticulum, conserved biosystem
• Function: electron carrier activity; isomerase activity; procollagen-proline 4-dioxygenase activity; protein disulfide isomerase activity; protein disulfide oxidoreductase activity; protein heterodimerization activity