Recombinant Mouse P4hb Protein, His-tagged

• Cat.No.: P4hb-7294M
• Product Overview: Recombinant Mouse P4hb protein with a His tag was expressed in insect cells.

Specification

• Description: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration.
• Source: Insect cell
• Species: Mouse
• Tag: His
• Form: Liquid
• Molecular Mass: 56.1 kDa
• Protein length: 20-509
• AA Sequence: DALEEEDNVLVLKKSNFEEALAAHK YLLVEFYAPWCGHCKALAPEYAKAA AKLKAEGSEIRLAKVDATEESDLAQ QYGVRGYPTIKFFKNGDTASPKEYT AGREADDIVNWLKKRTGPAATTLSD TAAAESLVDSSEVTVIGFFKDVESD SAKQFLLAAEAIDDIPFGITSNSGV FSKYQLDKDGVVLFKKFDEGRNNFE GEITKEKLLDFIKHNQLPLVIEFTE QTAPKIFGGEIKTHILLFLPKSVSD YDGKLSSFKRAAEGFKGKILFIFID SDHTDNQRILEFFGLKKEECPAVRL ITLEEEMTKYKPESDELTAEKITEF CHRFLEGKIKPHLMSQEVPEDWDKQ PVKVLVGANFEEVAFDEKKNVFVEF YAPWCGHCKQLAPIWDKLGETYKDH ENIIIAKMDSTANEVEAVKVHSFPT LKFFPASADRTVIDYNGERTLDGFK KFLESGGQDGAGDDEDLDLEEALEP DMEEDDDQKAVKDELLEHHHHHH
• Endotoxin: < 1.0 EU/μg of protein (determined by LAL method)
• Purity: > 95 % by SDS-PAGE
• Stability: Shelf life: one year from despatch.
• Storage: Store undiluted at 2-8 centigrade for one week or (in aliquots) at -20 to -80 centigrade for longer. Avoid repeated freezing and thawing.
• Concentration: 0.5 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate Buffered Saline (pH 7.4) containing 10 % glycerol.

Gene Information

• Gene Name: P4hb prolyl 4-hydroxylase, beta polypeptide [ Mus musculus (house mouse) ]
• Official Symbol: P4hb
• Synonyms: P4hb; prolyl 4-hydroxylase, beta polypeptide; P; Pd; ERp; PDI; Thb; Thbp; ERp59; Pdia1; protein disulfide-isomerase; ER protein 59; cellular thyroid hormone-binding protein; endoplasmic reticulum resident protein 59; p55; prolyl 4-hydroxylase subunit beta; EC 5.3.4.1
• Gene ID: 18453
• mRNA Refseq: NM_011032
• Protein Refseq: NP_035162
• UniProt ID: P09103