Recombinant Mouse Pcsk9, His-tagged, C13&N15 Labeled

• Cat.No.: Pcsk9-197M
• Product Overview: Recombinant Mouse PCSK9 MS Standard Protein, C13 and N15-labeled (Mouse PCSK9, Heavy Labeled) Gln 35 - Gln 694 (Accession # AAH38085.1) was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain >99% incorporation

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 35-694 a.a.
• Description: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
• Predicted N Terminal: Gln 35
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
• Molecular Mass: Mouse PCSK9, Heavy Labeled is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 72 kDa. The predicted N-terminus is Gln 35. DTT-reduced Protein migrates as 20 kDa and 64 kDa in SDS-PAGE due to glycosylation and proteolytic digesti
• Endotoxin: Less than 1.0 EU per μg of the Mouse PCSK9, Heavy Labeled by the LAL method.
• Purity: >97% as determined by SDS-PAGE.
• Applications: MS Standard Protein
• Storage: Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4oC); After reconstitution under sterile conditions for 3 months (-70oC).

Gene Information

• Gene Name: Pcsk9 proprotein convertase subtilisin/kexin type 9 [ Mus musculus ]
• Official Symbol: Pcsk9
• Synonyms: PCSK9; proprotein convertase subtilisin/kexin type 9; NARC-1; convertase subtilisin; proprotein convertase 9; proprotein convertase PC9; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; neural apoptosis-regulated convertase 1; FH3; PC9; Narc1; HCHOLA3; AI415265; AI747682; MGC47409
• Gene ID: 100102
• mRNA Refseq: NM_153565
• Protein Refseq: NP_705793
• Pathway: NGF processing, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signalling by NGF, organism-specific biosystem
• Function: apolipoprotein receptor binding; hydrolase activity; identical protein binding; low-density lipoprotein particle receptor binding; low-density lipoprotein particle receptor binding; peptidase activity; serine-type endopeptidase activity; serine-type endop