Recombinant Mouse Pcsk9 protein, His-tagged

• Cat.No.: Pcsk9-663M
• Product Overview: Recombinant Mouse Pcsk9 protein (Gln35-Gln694) was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Description: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily. Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
• Source: HEK293
• Species: Mouse
• Tag: His
• Predicted N Terminal: Gln35
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 72.0 kDa. The protein migrates as 17 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic digestion.
• Protein length: Gln35-Gln694
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >97% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Publication:
  MMP-2 inhibits PCSK9-induced degradation of the LDL receptor in Hepa1-c1c7 cells (2015)

Gene Information

• Gene Name: Pcsk9
• Official Symbol: Pcsk9
• Synonyms: PCSK9; proprotein convertase subtilisin/kexin type 9; NARC-1; convertase subtilisin; proprotein convertase 9; proprotein convertase PC9; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; neural apoptosis-regulated convertase 1; FH3; PC9; Narc1; HCHOLA3; AI415265; AI747682; MGC47409
• Gene ID: 100102
• mRNA Refseq: NM_153565
• Protein Refseq: NP_705793
• UniProt ID: Q80W65