"PDCD1" Related Products

Recombinant Mouse PDCD1 protein, mouse IgG2a Fc-tagged, low endotoxin

Cat.No. : PDCD1-369M
Product Overview : Recombinant Mouse PDCD1 protein (Ser21-Gln167)(low endotoxin), fused to Mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
Source : HEK293
Species : Mouse
Tag : Mouse IgG2a Fc
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 43.7 kDa. As a result of glycosylation, the protein migrates as 55-66 kDa under reducing (R) condition, and 100-130 kDa under non-reducing (NR) condition (SDS-PAGE).
Protein length : 147
Endotoxin : Less than 0.1 EU per ug by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of PDCD1-369M :

Mouse PD-1, mouse IgG2a Fc tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of PDCD1-369M :

Immobilized Mouse PD-L1, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse PD-1, Mouse IgG2a Fc Tag with a linear range of 0.078-0.313 μg/mL (QC tested).

Immobilized Mouse PD-L2, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse PD-1, Mouse IgG2a Fc Tag with a linear range of 0.078-0.625 μg/mL (Routinely tested).

Bioactivity-BLI of PDCD1-369M :

Loaded Human PD-L1, Fc Tag (HPLC-verified) on AHC Biosensor, can bind Mouse PD-1, Mouse IgG2a Fc Tag with an affinity constant of 378 nM as determined in BLI assay (Routinely tested).

Gene Name : PDCD1
Official Symbol : PDCD1
Synonyms : PDCD1; programmed cell death 1; programmed cell death protein 1; mPD-1; protein PD-1; PD-1; Pdc1; Ly101
Gene ID : 18566
mRNA Refseq : NM_008798
Protein Refseq : NP_032824
UniProt ID : Q544F3

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.