|Product Overview :||Recombinant Mouse PDCD1 protein (Ser21-Gln167)(low endotoxin), fused to Mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).|
|Description :||Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.|
|Tag :||Mouse IgG2a Fc|
|Form :||Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 43.7 kDa. As a result of glycosylation, the protein migrates as 55-66 kDa under reducing (R) condition, and 100-130 kDa under non-reducing (NR) condition (SDS-PAGE).|
|Protein length :||147|
|Endotoxin :||Less than 0.1 EU per ug by the LAL method.|
|Purity :||>90% as determined by SDS-PAGE.|
|Storage :||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of PDCD1-369M :||
Mouse PD-1, mouse IgG2a Fc tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
|Bioactivity-ELISA of PDCD1-369M :||
Immobilized Mouse PD-L1, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse PD-1, Mouse IgG2a Fc Tag with a linear range of 0.078-0.313 μg/mL (QC tested).
Immobilized Mouse PD-L2, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse PD-1, Mouse IgG2a Fc Tag with a linear range of 0.078-0.625 μg/mL (Routinely tested).
|Bioactivity-BLI of PDCD1-369M :||
Loaded Human PD-L1, Fc Tag (HPLC-verified) on AHC Biosensor, can bind Mouse PD-1, Mouse IgG2a Fc Tag with an affinity constant of 378 nM as determined in BLI assay (Routinely tested).
|Gene Name :||PDCD1|
|Official Symbol :||PDCD1|
|Synonyms :||PDCD1; programmed cell death 1; programmed cell death protein 1; mPD-1; protein PD-1; PD-1; Pdc1; Ly101|
|Gene ID :||18566|
|mRNA Refseq :||NM_008798|
|Protein Refseq :||NP_032824|
|UniProt ID :||Q544F3|