Recombinant Mouse Pdgfd Protein, Ser250-Arg370

• Cat.No.: PDGFD-12569M
• Product Overview: Recombinant Mouse PDGF-D Protein, Ser250-Arg370, without tag was expressed in Mouse myeloma cell line.

Specification

• Species: Mouse
• Source: Mouse myeloma cell
• Protein Length: Ser250-Arg370
• Description: Predicted to enable platelet-derived growth factor receptor binding activity. Acts upstream of or within cellular response to amino acid stimulus and regulation of peptidyl-tyrosine phosphorylation. Located in extracellular space. Is expressed in bladder; brain; and urethra of male. Human ortholog(s) of this gene implicated in prostatic hypertrophy. Orthologous to human PDGFD (platelet derived growth factor D).
• Form: Disulfide-linked homodimer
• Bio-activity: Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. The ED50 for this effect is 15-90 ng/mL
• Molecular Mass: Predicted Molecular Mass: 14 kDa
  SDS-PAGE: 18-20 kDa, reducing conditions
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• N-terminal Sequence Analysis: Ser250
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in HCl.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 250 μg/mL in 4 mM HCl.
• References: 1. Reigstad, L.J. et al. (2005) FEBS J. 272:5723.
  2. Fredriksson, L. et al. (2004) Cytokine Growth Factor Rev. 15:197.
  3. LaRochelle, W.J. et al. (2001) Nat. Cell Biol. 3:517.

Gene Information

• Gene Name: Pdgfd platelet-derived growth factor, D polypeptide [ Mus musculus ]
• Official Symbol: Pdgfd
• Synonyms: PDGFD; platelet-derived growth factor, D polypeptide; platelet-derived growth factor D; PDGF-D; SCDGF-B; spinal cord-derived growth factor B; 1110003I09Rik
• Gene ID: 71785
• mRNA Refseq: NM_027924
• Protein Refseq: NP_082200
• UniProt ID: Q925I7