Active Recombinant Mouse PDIA3 Protein, His-tagged

• Cat.No.: PDIA3-32M
• Product Overview: Recombinant Mouse PDIA3 Protein (25-505aa) with His tag was expressed in E.coli.

Specification

• Description: Protein disulfide-isomerase A3 (PDIA3), also known as glucose-regulated protein, 58-kD (GRP58), is an isomerase enzyme. This protein localizes to the endoplasmic reticulum (ER) and interacts with lectin chaperones calreticulin and calnexin (CNX) to modulate folding of newly synthesized glycoproteins. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity is >15 A650/cm/min/mg,obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin.
• Molecular Mass: 56.8kDa (505aa), confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Applications: Enzyme Activity, SDS-PAGE
• Notes: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)
• Storage Buffer: In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1M NaCl, and 10% glycerol

Gene Information

• Gene Name: Pdia3 protein disulfide isomerase associated 3 [ Mus musculus (house mouse) ]
• Official Symbol: Pdia3
• Synonyms: Pdia3; protein disulfide isomerase associated 3; Erp; PDI; Plca; 58kDa; ERp57; ERp60; ERp61; Grp58; PDI-Q2; PI-PLC; PLC[a]; protein disulfide-isomerase A3; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; ER protein 57; ER protein 60; disulfide isomerase ER-60; endoplasmic reticulum protein; endoplasmic reticulum resident protein 57; endoplasmic reticulum resident protein 60; glucose regulated protein, 58 kDa; p58; phospholipase C, alpha; EC 5.3.4.1
• Gene ID: 14827
• mRNA Refseq: NM_007952
• Protein Refseq: NP_031978
• UniProt ID: P27773