Recombinant Mouse Pdk1 protein, His & T7-tagged

• Cat.No.: Pdk1-8026M
• Product Overview: Recombinant Mouse Pdk1 aa. (Val172~Pro399 (Accession # Q8BFP9)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.7kDa
• Protein length: Val172~Pro399 (Accession # Q8BFP9)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 8.4.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Pdk1 pyruvate dehydrogenase kinase, isoenzyme 1 [ Mus musculus (house mouse) ]
• Official Symbol: Pdk1
• Synonyms: B830012B01; D530020C15Rik; PDH kinase 1; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; pyruvate dehydrogenase kinase, isozyme 1
• Gene ID: 228026
• mRNA Refseq: NM_172665.5
• Protein Refseq: NP_766253.2
• UniProt ID: Q8BFP9

Reviews

07/22/2022

PDK1 production process is strictly controlled and the quality of the product is guaranteed.

08/08/2020

Reproducibility of PDK1 is simply unrivaled, with identical results in every experiment or application, demonstrating its high stability and reliability.

09/15/2019

The catalytic activity of PDK1 is excellent, which can simplify complex chemical reactions and improve yields, and its scientific research value is self-evident.

Q&As

Is PDK1 associated with obesity?

May play a role in the occurrence of obesity, and the current research conclusions still need to be further verified.

Is PDK1 related to the cardiovascular system?

PDK1 is thought to be associated with the occurrence and progression of cardiovascular disease, and may affect the health of the cardiovascular system by regulating the glucose metabolism of cardiomyocytes and the activity of phosphatase. However, further research and validation are needed.

What signal pathways does PDK1 participate in and how does it regulate signals?

Participates in a variety of signaling pathways, such as Akt and PKC, and affects the transmission and regulation of various signaling pathways by regulating the kinase activities of related receptors and enzymes.

In which tumors are PDK1 abnormally expressed?

PDK1 has abnormal expression in a variety of tumors, such as breast cancer, pancreatic cancer, colorectal cancer, etc., and is one of the important markers of tumor occurrence and prognosis.

How does PDK1 regulate cell metabolism?

The plays an important role in regulating cell metabolism and plays an important role in energy metabolism by regulating the phosphorylation state of various enzymes.

Can PDK1 be used as a target for the treatment of new diabetes?

It has been identified as a potential therapeutic target for new diabetes, possibly by improving insulin signaling and glycogen synthesis, but further exploration is needed.