Recombinant Mouse Phlpp2 Protein, Myc/DDK-tagged

• Cat.No.: Phlpp2-4843M
• Product Overview: Purified recombinant protein of mouse full-length PH domain and leucine rich repeat protein phosphatase 2 (Phlpp2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Protein phosphatase involved in regulation of Akt and PKC signaling. Mediates dephosphorylation in the C-terminal domain hydrophobic motif of members of the AGC Ser/Thr protein kinase family; specifically acts on 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. Akt regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of Akt triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylates STK4 on 'Thr-387' leading to STK4 activation and apoptosis. Dephosphorylates RPS6KB1 and is involved in regulation of cap-dependent translation. Inhibits cancer cell proliferation and may act as a tumor suppressor. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Dephosphorylates RAF1 inhibiting its kinase activity.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 150.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Phlpp2 PH domain and leucine rich repeat protein phosphatase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Phlpp2
• Synonyms: PHLPP2; PH domain and leucine rich repeat protein phosphatase 2; PH domain leucine-rich repeat-containing protein phosphatase 2; GTPase activating protein and VPS9 domains 1; Phlppl; AI481772; C130044A18Rik; KIAA0931
• Gene ID: 244650
• mRNA Refseq: NM_001122594
• Protein Refseq: NP_001116066
• UniProt ID: Q8BXA7