Recombinant Mouse PITX3 Protein (1-302 aa), GST-tagged

• Cat.No.: PITX3-720M
• Product Overview: Recombinant Mouse PITX3 Protein (1-302 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Protein Description: Full Length.

Specification

• Description: Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the tporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle.
• Source: E. coli
• Species: Mouse
• Tag: GST
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 58.7 kDa
• Protein length: 1-302 aa
• AA Sequence: MEFGLLGEAEARSPALSLSDAGTPH PPLPEHGCKGQEHSDSEKASASLPG GSPEDGSLKKKQRRQRTHFTSQQLQ ELEATFQRNRYPDMSTREEIAVWTN LTEARVRVWFKNRRAKWRKRERSQQ AELCKGGFAAPLGGLVPPYEEVYPG YSYGNWPPKALAPPLAAKTFPFAFN SVNVGPLASQPVFSPPSSIAASMVP SAAAAPGTVPGPGALQGLGGAPPGL APAAVSSGAVSCPYASAAAAAAAAA SSPYVYRDPCNSSLASLRLKAKQHA SFSYPAVPGPPPAANLSPCQYAVER PV
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: Pitx3 paired-like homeodomain transcription factor 3 [ Mus musculus ]
• Official Symbol: PITX3
• Synonyms: PITX3; aphakia; ak; Ptx3
• Gene ID: 18742
• mRNA Refseq: NM_008852
• Protein Refseq: NP_032878
• UniProt ID: O35160