Recombinant Mouse Pkmyt1 Protein, Myc/DDK-tagged

• Cat.No.: Pkmyt1-4889M
• Product Overview: Purified recombinant protein of mouse full-length protein kinase, membrane associated tyrosine/threonine 1 (Pkmyt1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 54.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pkmyt1 protein kinase, membrane associated tyrosine/threonine 1 [ Mus musculus (house mouse) ]
• Official Symbol: Pkmyt1
• Synonyms: PKMYT1; protein kinase, membrane associated tyrosine/threonine 1; membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; MYT1 kinase; membrane-associated tyrosine-and threonine-specific cdc2-inhibitory kinase; Myt1; AW209059; 6230424P17
• Gene ID: 268930
• mRNA Refseq: NM_023058
• Protein Refseq: NP_075545
• UniProt ID: Q9ESG9