Recombinant Mouse PLA2G15 Protein (34-412 aa), His-Myc-tagged

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Recombinant Mouse PLA2G15 Protein (34-412 aa), His-Myc-tagged

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Cat.No. : PLA2G15-2522M
Product Overview : Recombinant Mouse PLA2G15 Protein (34-412 aa) is produced by E. coli expression system. This protein is fused with a 10xHis tag at the N-terminal and a Myc tag at the C-terminal. Research Area: Epigenetics and Nuclear Signaling. Protein Description: Full Length of Mature Protein.
Description : Has transacylase and calcium-independent phospholipase A2 activity. Catalyzes the formation of 1-O-acyl-N-acetylsphingosine and the concomitant release of a lyso-phospholipid. Has high activity with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), catalyzing the transfer of oleic acid to N-acetyl-sphingosine. Required for normal phospholipid degradation in alveolar and peritoneal macrophages and in spleen.
Source : E. coli
Species : Mouse
Tag : His-Myc
Form : Tris-based buffer,50% glycerol
Molecular Mass : 50.5 kDa
Protein length : 34-412 aa
AA Sequence : AQRHPPVVLV PGDLGNQLEA KLDKPKVVHY LCSKKTDSYF TLWLNLELLL PVIIDCWIDN IRLVYNRTSR ATQFPDGVDV RVPGFGETFS MEFLDPSKRN VGSYFYTMVE SLVGWGYTRG EDVRGAPYDW RRAPNENGPY FLALREMIEE MYQMYGGPVV LVAHSMGNVY MLYFLQRQPQ VWKDKYIHAF VSLGAPWGGV AKTLRVLASG DNNRIPVIGP LKIREQQRSA VSTSWLLPYN HTWSHEKVFV YTPTTNYTLR DYHRFFRDIG FEDGWFMRQD TEGLVEAMTP PGVELHCLYG TGVPTPNSFY YESFPDRDPK ICFGDGDGTV NLESVLQCQA WQSRQEHRVS LQELPGSEHI EMLANATTLA YLKRVLLEP
Purity : > 85% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : Pla2g15 phospholipase A2, group XV [ Mus musculus ]
Official Symbol : PLA2G15
Synonyms : PLA2G15; lysophospholipase 3; 1-O-acylceramide synthase; ACS; LLPL; Lpla2; C87498; Lypla3;
Gene ID : 192654
mRNA Refseq : NM_133792
Protein Refseq : NP_598553
UniProt ID : Q8VEB4

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