Recombinant Mouse Pla2g4a Protein, Myc/DDK-tagged

• Cat.No.: Pla2g4a-4901M
• Product Overview: Purified recombinant protein of mouse full-length phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: The protein encoded by this gene is a member of the phospholipase A2 group IV family. This enzyme hydrolyzes membrane phospholipids, thereby releasing the polyunsaturated fatty acid, arachidonic acid. Arachidonic acid is further metabolized into eicosanoids such as leukotrienes, thromboxanes and prostaglandins, that play important roles in regulating diverse biological processes such as inflammatory responses, membrane and actin dynamics, and tumorigenesis. A rise in intracellular calcium levels results in binding of calcium to the C2 domain of this protein, and triggers the translocation from the cytosol to intracellular membranes, including the Golgi apparatus. Disruption of this gene in mice led to decreased levels of eicosonaoids and platelet-activating factor, decreased allergic symptoms, and impaired reproductive ability in females. Alternative splicing results in multiple transcript variants encoding different isoforms.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 85.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Pla2g4a phospholipase A2, group IVA (cytosolic, calcium-dependent) [ Mus musculus (house mouse) ]
• Official Symbol: Pla2g4a
• Synonyms: PLA2G4A; phospholipase A2, group IVA (cytosolic, calcium-dependent); cytosolic phospholipase A2; cPLA2-alpha; Type IV PLA2; cytosolic PLA2; phospholipase A2, group 4; phospholipase A2 group IVA; Cytosolic phospholipase A2 (CPLA2); cPLA2; Pla2g4; cPLA2alpha
• Gene ID: 18783
• mRNA Refseq: NM_008869
• Protein Refseq: NP_032895
• UniProt ID: P47713