Recombinant Mouse Plekhm1 Protein, Myc/DDK-tagged

• Cat.No.: Plekhm1-4932M
• Product Overview: Purified recombinant protein of mouse full-length pleckstrin homology domain containing, family M (with RUN domain) member 1 (Plekhm1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Proposed to act as a multivalent adapter protein that regulates Rab7-dependent and HOPS complex-dependent fusion events in the endolysosomal system and couples autophagic and the endocytic trafficking pathways. Required for late stages of endolysosomal maturation, facilitating both endocytosis-mediated degradation of growth factor receptors and autophagosome clearance. Seems to be involved in the terminal maturation of autophagosomes and to mediate autophagosome-lysosome fusion Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts May be involved in negative regulation of endocytic transport from early endosome to late endosome/lysosome implicating its association with Rab7. May have a role in sialyl-lex-mediated transduction of apoptotic signals. Involved in bone resorption
• Molecular Mass: 118.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Plekhm1 pleckstrin homology domain containing, family M (with RUN domain) member 1 [ Mus musculus (house mouse) ]
• Official Symbol: Plekhm1
• Synonyms: PLEKHM1; pleckstrin homology domain containing, family M (with RUN domain) member 1; pleckstrin homology domain-containing family M member 1; PH domain-containing family M member 1; B2; AP162; BC038943; D330036J23Rik
• Gene ID: 353047
• mRNA Refseq: NM_183034
• Protein Refseq: NP_898855
• UniProt ID: Q7TSI1