Recombinant Mouse Pold1 Protein, His-tagged

• Cat.No.: Pold1-1958M
• Product Overview: Recombinant Mouse Pold1 Protein (Ser29-Gly260) with N-His tag was expressed in E. coli.

Specification

• Description: Enables 3'-5' exonuclease activity and enzyme binding activity. Involved in DNA biosynthetic process. Acts upstream of or within DNA replication proofreading and base-excision repair, gap-filling. Part of delta DNA polymerase complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; respiratory system; and sensory organ. Orthologous to human POLD1 (DNA polymerase delta 1, catalytic subunit).
• Source: E. coli
• Species: Mouse
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 29.1 kDa
  Accurate Molecular Mass: 30 kDa
• Protein length: Ser29-Gly260
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Pold1 polymerase (DNA directed), delta 1, catalytic subunit [ Mus musculus (house mouse) ]
• Official Symbol: Pold1
• Synonyms: Pold1; polymerase (DNA directed), delta 1, catalytic subunit; 125kDa; DNA polymerase delta catalytic subunit; 3'-5' exodeoxyribonuclease; DNA polymerase delta 1, catalytic domain; EC 2.7.7.7
• Gene ID: 18971
• mRNA Refseq: NM_011131
• Protein Refseq: NP_035261
• UniProt ID: P52431