Recombinant Mouse Prmt7 Protein, Myc/DDK-tagged

• Cat.No.: Prmt7-5135M
• Product Overview: Purified recombinant protein of mouse full-length protein arginine N-methyltransferase 7 (Prmt7), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 78.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Prmt7 protein arginine N-methyltransferase 7 [ Mus musculus (house mouse) ]
• Official Symbol: Prmt7
• Synonyms: PRMT7; protein arginine N-methyltransferase 7; histone-arginine N-methyltransferase PRMT7; [Myelin basic protein]-arginine N-methyltransferase PRMT7; BC006705; MGC7929; 4933402B05Rik
• Gene ID: 214572
• mRNA Refseq: NM_145404
• Protein Refseq: NP_663379
• UniProt ID: Q922X9