Recombinant Mouse PTPN6 cell lysate
Cat.No. : | PTPN6-001MCL |
Product Overview : | Mouse PTPN6 (aa 207-597) derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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- Gene Information
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Source : | Baculovirus-Insect Cells |
Species : | Mouse |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Gene Name : | Ptpn6 protein tyrosine phosphatase, non-receptor type 6 [ Mus musculus ] |
Official Symbol : | PTPN6 |
Synonyms : | PTPN6; protein tyrosine phosphatase, non-receptor type 6; tyrosine-protein phosphatase non-receptor type 6; PTP-1C; 70Z-SHP; SH-PTP1; PTPTY-42; hemopoietic cell phosphatase; protein-tyrosine phosphatase 1C; hematopoietic cell protein-tyrosine phosphatase; me; hcp; Hcph; Ptp1C; SHP-1; motheaten; |
Gene ID : | 15170 |
mRNA Refseq : | NM_001077705 |
Protein Refseq : | NP_001071173 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; B cell receptor signaling pathway, organism-specific biosystem; B cell receptor signaling pathway, conserved biosystem; Cell-Cell communication, organism-specific biosystem; |
Function : | SH2 domain binding; SH3 domain binding; hydrolase activity; natural killer cell lectin-like receptor binding; phosphatase activity; phosphoprotein phosphatase activity; phosphotyrosine binding; protein binding; protein kinase binding; protein tyrosine phosphatase activity; transmembrane receptor protein tyrosine phosphatase activity; |
Products Types
◆ Recombinant Protein | ||
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PTPN6-1802H | Recombinant Human PTPN6 Protein, His (Fc)-Avi-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionPTPN6 acts as a phosphatase, removing phosphate groups from specific tyrosine residues in signaling molecules, thereby downregulating immune cell activation.
Yes, PTPN6 modulation is being explored as a potential target for immunotherapies aimed at enhancing or suppressing immune responses based on the clinical context.
PTPN6 negatively regulates T cell receptor signaling, and its modulation can have implications in controlling excessive immune responses seen in autoimmune diseases.
Yes, PTPN6 is being studied in diseases like rheumatoid arthritis, lupus, and certain types of leukemia and lymphoma.
Researchers are exploring small molecule inhibitors and gene therapies to modulate PTPN6 activity, with potential applications in autoimmune disorders and cancer treatment.
Customer Reviews (3)
Write a reviewStructural biology researchers can leverage its properties to better understand the three-dimensional architecture and conformational changes of proteins, gaining valuable insights into their function and interactions.
PTPN6 protein is highly recommended for various research applications, including ELISA and protein electron microscopy structure analysis.
PTPN6 protein might have biomarker properties, meaning its levels or activity could be indicative of disease progression or treatment response.
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