| Description : |
CD112 (also called Nectin-2 or poliovirus receptor-related 2 protein) is a type I transmembrane glycoprotein and a member of the Ig gene superfamily (IGSF). CD112/Nectin-2 functions as an adhesion molecule involved in the formation of cell junctions and interactions with other Nectin-family molecules. Nectin-2 is a also a surface marker of some endothelial cells and regulates migration, proliferation and angiogenesis. CD112 functions as an immune checkpoint regulator by modulating T cell signaling, either as a costimulator of T cell function, or as a coinhibitor of T cells proliferation by binding to the receptors CD226 or TIGIT (T cell Ig and immunoreceptor tyrosine-based inhibitory motif (ITIM) domain), respectively. The binding of CD112 ligand to these receptors is weak compared to CD155. Recently, a new receptor of CD112, called CD112R or PVRIG (Poliovirus receptor-related immunoglobulin domain-containing protein) has been characterized, showing high affinity to CD112. CD112R is a new coinhibitory receptor of the PVR family for human T cells, inhibiting TCR-mediated signals upon binding to CD112/Nectin-2. CD112R is preferentially expressed in T lymphocytes and NK cells. Cancer cells evade immune responses as a result of an immunosuppressive tumor microenvironment and CD112R, by playing a role in the regulation of the immune system, could be a new attractive cancer immunotherapy target. |
| Source : |
HEK293 cells |
| Species : |
Mouse |
| Tag : |
Fc |
| Form : |
Lyophilized. Contains PBS. |
| Bio-activity : |
Binds to the ligand mouse CD112 (PVRL2, Nectin-2) with an EC50 of 5nM. |
| Molecular Mass : |
~48-50 kDa (SDS-PAGE) |
| Endotoxin : |
<0.01EU/μg purified protein (LAL test) |
| Purity : |
>90% (SDS-PAGE) |
| Stability : |
Stable for at least 6 months after receipt when stored at -20 centigrade. |
| Storage : |
Short term store at +4 centigrade, store at -20 centigrade for long term storage. |
| Concentration : |
0.1mg/mL after reconstitution. |
| Reconstitution : |
Reconstitute with 100 μL sterile water. |
| Handling : |
After reconstitution, prepare aliquots and store at -20 centigrade. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions. |
