Recombinant Mouse Rac1 Protein, Myc/DDK-tagged

• Cat.No.: Rac1-5344M
• Product Overview: Purified recombinant protein of mouse full-length Rac family small GTPase 1 (Rac1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization, neurons adhesion, migration and differentiation, and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. In neurons, is involved in dendritic spine formation and synaptic plasticity. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 21.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rac1 Rac family small GTPase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Rac1
• Synonyms: RAC1; RAS-related C3 botulinum substrate 1; ras-related C3 botulinum toxin substrate 1; p21-Rac1; AL023026; D5Ertd559e
• Gene ID: 19353
• mRNA Refseq: NM_009007
• Protein Refseq: NP_033033
• UniProt ID: P63001