Recombinant Mouse RIPK1 Protein (1-656 aa), His-SUMOSTAR-tagged

• Cat.No.: RIPK1-2252M
• Product Overview: Recombinant Mouse RIPK1 Protein (1-656 aa) is produced by Yeast expression system. This protein is fused with a 6xHis-SUMOSTAR tag at the N-terminal. Research Area: Cell Biology. Protein Description: Full Length.

Specification

• Description: Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex. Interacts with ARHGEF2.
• Source: Yeast
• Species: Mouse
• Tag: His-SUMOSTAR
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 88 kDa
• Protein length: 1-656 aa
• AA Sequence: MQPDMSLDNIKMASSDLLEKTDLDS GGFGKVSLCYHRSHGFVILKKVYTG PNRAEYNEVLLEEGKMMHRLRHSRV VKLLGIIIEEGNYSLVMEYMEKGNL MHVLKTQIDVPLSLKGRIIVEAIEG MCYLHDKGVIHKDLKPENILVDRDF HIKIADLGVASFKTWSKLTKEKDNK QKEVSSTTKKNNGGTLYYMAPEHLN DINAKPTEKSDVYSFGIVLWAIFAK KEPYENVICTEQFVICIKSGNRPNV EEILEYCPREIISLMERCWQAIPED RPTFLGIEEEFRPFYLSHFEEYVEE DVASLKKEYPDQSPVLQRMFSLQHD CVPLPPSRSNSEQPGSLHSSQGLQM GPVEESWFSSSPEYPQDENDRSVQA KLQEEASYHAFGIFAEKQTKPQPRQ NEAYNREEERKRRVSHDPFAQQRAR ENIKSAGARGHSDPSTTSRGIAVQQ LSWPATQTVWNNGLYNQHGFGTTGT GVWYPPNLSQMYSTYKTPVPETNIP GSTPTMPYFSGPVADDLIKYTIFNS SGIQIGNHNYMDVGLNSQPPNNTCK EESTSRHQAIFDNTTSLTDEHLNPI RENLGRQWKNCARKLGFTESQIDEI DHDYERDGLKEKVYQMLQKWLMREG TKGATVGKLAQALHQCCRIDLLNHL IRASQS
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: Ripk1 receptor (TNFRSF)-interacting serine-threonine kinase 1 [ Mus musculus ]
• Official Symbol: RIPK1
• Synonyms: RIPK1; RIP-1; cell death protein RIP; RIP; Rinp; Rip1; D330015H01Rik
• Gene ID: 19766
• mRNA Refseq: NM_009068
• Protein Refseq: NP_033094
• UniProt ID: Q60855