Recombinant Mouse Rps6kb1 Protein, Myc/DDK-tagged

• Cat.No.: Rps6kb1-5613M
• Product Overview: Purified recombinant protein of mouse full-length ribosomal protein S6 kinase, polypeptide 1 (Rps6kb1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR. Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 59.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rps6kb1 ribosomal protein S6 kinase, polypeptide 1 [ Mus musculus (house mouse) ]
• Official Symbol: Rps6kb1
• Synonyms: RPS6KB1; ribosomal protein S6 kinase, polypeptide 1; ribosomal protein S6 kinase beta-1; S6K; P70S6K1; p70 S6KA; p70-S6K 1; S6K-beta-1; p70 S6K-alpha; p70 S6 kinase alpha; p70 ribosomal S6 kinase alpha; ribosomal protein S6 kinase I; 70 kDa ribosomal protein S6 kinase 1; ribosomal protein S6 kinase, 70kD, polypeptide 1; S6K1; 70kDa; p70s6k; AA959758; AI256796; AI314060; p70/85s6k; 2610318I15Rik; 4732464A07Rik
• Gene ID: 72508
• mRNA Refseq: NM_001114334
• Protein Refseq: NP_001107806
• UniProt ID: Q8BSK8