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Recombinant Mouse Rps6kb1 Protein, Myc/DDK-tagged

Cat.No. : Rps6kb1-5613M
Product Overview : Purified recombinant protein of mouse full-length ribosomal protein S6 kinase, polypeptide 1 (Rps6kb1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.
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Description : Serine/threonine-protein kinase that acts downstream of mTOR signaling in response to growth factors and nutrients to promote cell proliferation, cell growth and cell cycle progression. Regulates protein synthesis through phosphorylation of EIF4B, RPS6 and EEF2K, and contributes to cell survival by repressing the pro-apoptotic function of BAD. Under conditions of nutrient depletion, the inactive form associates with the EIF3 translation initiation complex. Upon mitogenic stimulation, phosphorylation by the mammalian target of rapamycin complex 1 (mTORC1) leads to dissociation from the EIF3 complex and activation. The active form then phosphorylates and activates several substrates in the pre-initiation complex, including the EIF2B complex and the cap-binding complex component EIF4B. Also controls translation initiation by phosphorylating a negative regulator of EIF4A, PDCD4, targeting it for ubiquitination and subsequent proteolysis. Promotes initiation of the pioneer round of protein synthesis by phosphorylating POLDIP3/SKAR. In response to IGF1, activates translation elongation by phosphorylating EEF2 kinase (EEF2K), which leads to its inhibition and thus activation of EEF2. Also plays a role in feedback regulation of mTORC2 by mTORC1 by phosphorylating RICTOR, resulting in the inhibition of mTORC2 and AKT1 signaling. Mediates cell survival by phosphorylating the pro-apoptotic protein BAD and suppressing its pro-apoptotic function. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex. The free mitochondrial PPP1CC can then dephosphorylate RPS6KB1 at Thr-412, which is proposed to be a negative feedback mechanism for the RPS6KB1 anti-apoptotic function. Mediates TNF-alpha-induced insulin resistance by phosphorylating IRS1 at multiple serine residues, resulting in accelerated degradation of IRS1. In cells lacking functional TSC1-2 complex, constitutively phosphorylates and inhibits GSK3B. May be involved in cytoskeletal rearrangement through binding to neurabin. Phosphorylates and activates the pyrimidine biosynthesis enzyme CAD, downstream of MTOR. Following activation by mTORC1, phosphorylates EPRS and thereby plays a key role in fatty acid uptake by adipocytes and also most probably in interferon-gamma-induced translation inhibition.
Source : HEK293T
Species : Mouse
Tag : Myc/DDK
Molecular Mass : 59.1 kDa
Purity : > 80% as determined by SDS-PAGE and Coomassie blue staining
Stability : Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Storage : Store at -80 centigrade after receiving vials.
Concentration : >50 μg/mL as determined by microplate BCA method
Storage Buffer : 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
Gene Name : Rps6kb1 ribosomal protein S6 kinase, polypeptide 1 [ Mus musculus (house mouse) ]
Official Symbol : Rps6kb1
Synonyms : RPS6KB1; ribosomal protein S6 kinase, polypeptide 1; ribosomal protein S6 kinase beta-1; S6K; P70S6K1; p70 S6KA; p70-S6K 1; S6K-beta-1; p70 S6K-alpha; p70 S6 kinase alpha; p70 ribosomal S6 kinase alpha; ribosomal protein S6 kinase I; 70 kDa ribosomal protein S6 kinase 1; ribosomal protein S6 kinase, 70kD, polypeptide 1; S6K1; 70kDa; p70s6k; AA959758; AI256796; AI314060; p70/85s6k; 2610318I15Rik; 4732464A07Rik
Gene ID : 72508
mRNA Refseq : NM_001114334
Protein Refseq : NP_001107806
UniProt ID : Q8BSK8

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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What are the mechanisms by which RPS6KB1 modulates autophagy and cell survival? 04/14/2022

RPS6KB1 regulates autophagy and cell survival, crucial for cellular health and response to stress.

How does RPS6KB1 (Ribosomal Protein S6 Kinase B1) regulate protein synthesis and cell growth? 02/22/2021

RPS6KB1 controls protein synthesis and cell growth, playing a key role in cellular proliferation.

What is the impact of RPS6KB1 on cellular responses to stress and DNA damage? 05/22/2020

RPS6KB1 impacts cellular stress responses, including the repair of DNA damage and maintenance of genomic stability.

How does RPS6KB1 contribute to the development and progression of cancer? 01/07/2020

RPS6KB1 can contribute to cancer by promoting tumor cell growth and proliferation.

What role does RPS6KB1 play in the mTOR signaling pathway, particularly in response to nutrient availability? 08/26/2019

RPS6KB1 is a critical component of the mTOR pathway, responding to nutrient signals to regulate cell metabolism and growth.

How do alterations in RPS6KB1 expression or activity affect aging and age-related diseases? 03/05/2019

Changes in RPS6KB1 function can affect aging processes and the development of age-related diseases.

How does RPS6KB1 influence metabolic processes, especially in conditions like obesity and diabetes? 02/13/2018

RPS6KB1 influences metabolic processes, affecting how cells respond to conditions like obesity and diabetes.

Customer Reviews (3)

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Reviews
09/16/2021

    Dependable protein purification, consistently high-quality results.

    06/11/2021

      Precise mass spectrometry analysis, essential for our proteomics work.

      06/30/2019

        Efficient peptide synthesis, crucial for our peptide research projects.

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