Recombinant Mouse Rtcb Protein, Myc/DDK-tagged

• Cat.No.: Rtcb-5639M
• Product Overview: Purified recombinant protein of mouse full-length RNA 2',3'-cyclic phosphate and 5'-OH ligase (Rtcb), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs. Essential during post-implantation development of embryos.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 55.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Rtcb RNA 2',3'-cyclic phosphate and 5'-OH ligase [ Mus musculus (house mouse) ]
• Official Symbol: Rtcb
• Synonyms: Rtcb; RNA 2',3'-cyclic phosphate and 5'-OH ligase; FAAP; HSPC1; HSPC117; AI255213; AI463255; D10Wsu52; D10Wsu52e; RNA-splicing ligase RtcB homolog; 3'-phosphate/5'-hydroxy nucleic acid ligase; focal adhesion-associated protein; tRNA-splicing ligase RtcB homolog; EC 6.5.1.8
• Gene ID: 28088
• mRNA Refseq: NM_145422
• Protein Refseq: NP_663397
• UniProt ID: Q99LF4