Recombinant Mouse S100b protein(Ser2-Glu92), hFc-tagged

• Cat.No.: S100b-7412M
• Product Overview: Recombinant Mouse S100B (P50114) (Ser2-Glu92) was expressed in HEK293 with the fused Fc region of Human IgG1 at the N-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 2-92 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse S100B/Fc comprises 351 amino acids and has a predicted molecular mass of 39 kDa. The apparent molecular mass of the protein is approximately 41 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S100b S100 protein, beta polypeptide, neural [ Mus musculus ]
• Official Symbol: S100b
• Synonyms: S100B; S100 protein, beta polypeptide, neural; protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; Bpb; AI850290; MGC74317
• Gene ID: 20203
• mRNA Refseq: NM_009115
• Protein Refseq: NP_033141

Citation

The receptor for advanced glycation endproducts mediates podocyte heparanase expression through NF-κB signaling pathway.

Journal: Molecular and cellular endocrinology    PubMed ID: 28478303    Data: 2019/3/22

Authors: Xiaofei An, Lin Zhang, Jinan Zhang

Article Snippet:Nε-(carboxymethyl) lysine (CML)-BSA (Cell Biolabs, San Diego, CA, USA), mouse HMBG-1 protein (Thermo Scientific, Shanghai, China) and mouse S100B protein (Creative BioMart, Shirley, NY, USA) were diluted in PBS at different concentration.. TPP488, PDTC, SB203580 and LY294002 used for cell experiment were from Sigma Corporation (Hong Kong, China) and dissolved in 0.1% DMSO.TPP488, PDTC, SB203580 and LY294002 used for cell experiment were from Sigma Corporation (Hong Kong, China) and dissolved in 0.1% DMSO.