Recombinant Mouse Sag protein, His & T7-tagged Sag-1962M

Recombinant Mouse Sag protein, His & T7-tagged

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Recombinant Mouse Sag protein, His & T7-tagged

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Cat.No. : Sag-1962M
Product Overview : Recombinant Mouse Sag aa. (Cys4~Val249 (Accession # P20443)) fused with N-terminal His & T7 tag was produced in E. coli cells.
Source : E. coli
Species : Mouse
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 31.3kDa
Protein length : Cys4~Val249 (Accession # P20443)
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : > 95%
Characteristic : The isoelectric point is 8.5.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage Buffer : Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Reconstitution : Reconstitute in sterile PBS, pH7.2-pH7.4.
Figure :

SDS-PAGE

Gene Name : Sag S-antigen, retina and pineal gland (arrestin) [ Mus musculus (house mouse) ]
Official Symbol : Sag
Synonyms : 1500019G21Rik; SPA ) binding protein, cytoplasmic cochaperone 1; heat shock protein-binding protein 1; hsp70-interacting protein 1; hsp70-binding protein 1
Gene ID : 20215
mRNA Refseq : NM_009118.2
Protein Refseq : NP_033144.1
UniProt ID : P20443

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