Active Recombinant Mouse Sele protein, hFc&His-tagged

• Cat.No.: Sele-1720M
• Product Overview: Recombinant Mouse Sele protein(Trp22-Pro557), fused with C-terminal hFc and His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc&His
• Protein Length: 22-557 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse E-Selectin coated plates (2 μg/mL with 100 μL/well), approximately 80%-90% cells will adhere for 1 hour incubation at 37°C.
• Molecular Mass: 85.26 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Sele selectin, endothelial cell [ Mus musculus ]
• Official Symbol: Sele
• Synonyms: SELE; selectin, endothelial cell; E-selectin; ELAM-1; LECAM2; CD62 antigen-like family member E; endothelial leukocyte adhesion molecule 1; leukocyte-endothelial cell adhesion molecule 2; Elam; CD62E
• Gene ID: 20339
• mRNA Refseq: NM_011345
• Protein Refseq: NP_035475