Recombinant Mouse Sema4d Protein, Myc/DDK-tagged

• Cat.No.: Sema4d-5760M
• Product Overview: Purified recombinant protein of mouse full-length sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D (Sema4d), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development. Promotes the development of inhibitory synapses in a PLXNB1-dependent manner. Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 95.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Sema4d sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D [ Mus musculus (house mouse) ]
• Official Symbol: Sema4d
• Synonyms: SEMA4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; semaphorin-4D; sema J; M-sema G; semaphorin H; semaphorin-J; semaphorin-C-like 2; CD100; Semaj; Semcl2; coll-4; Semacl2
• Gene ID: 20354
• mRNA Refseq: NM_013660
• Protein Refseq: NP_038688
• UniProt ID: O09126