Recombinant Mouse Setd7 Protein, Myc/DDK-tagged

• Cat.No.: Setd7-5801M
• Product Overview: Purified recombinant protein of mouse full-length SET domain containing (lysine methyltransferase) 7 (Setd7), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 40.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Setd7 SET domain containing (lysine methyltransferase) 7 [ Mus musculus (house mouse) ]
• Official Symbol: Setd7
• Synonyms: SETD7; SET domain containing (lysine methyltransferase) 7; histone-lysine N-methyltransferase SETD7; H3-K4-HMTase SETD7; H3-K4 methyltransferase; SET domain-containing protein 7; histone H3-K4 methyltransferase SETD7; KMT7; Set7; H3K4MT; Set7/9; mKIAA1717; 1600028F23Rik
• Gene ID: 73251
• mRNA Refseq: NM_080793
• Protein Refseq: NP_542983
• UniProt ID: Q8VHL1