Recombinant Mouse Sf3b5 Protein, Myc/DDK-tagged

• Cat.No.: Sf3b5-5811M
• Product Overview: Purified recombinant protein of mouse full-length splicing factor 3b, subunit 5 (Sf3b5), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex, a constituent of the spliceosome. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 10.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Sf3b5 splicing factor 3b, subunit 5 [ Mus musculus (house mouse) ]
• Official Symbol: Sf3b5
• Synonyms: SF3B5; splicing factor 3b, subunit 5; splicing factor 3B subunit 5; pre-mRNA-splicing factor SF3b 10 kDa subunit; 10kDa; Sf3b10; AU043053; 1110005L13Rik
• Gene ID: 66125
• mRNA Refseq: NM_175102
• Protein Refseq: NP_780311
• UniProt ID: Q923D4