Active Recombinant Mouse Sirpa protein, hFc-tagged

• Cat.No.: SIRPA-936M
• Product Overview: Recombinant Mouse Sirpa protein(Lys32-Asn373), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 32-373 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized Human CD47 at 2 μg/mL (100 μL/well) can bind Mouse CD172a with a linear range of 1.2-55.3 ng/mL.
• Molecular Mass: 63.86 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Sirpa signal-regulatory protein alpha [ Mus musculus ]
• Official Symbol: Sirpa
• Synonyms: SIRPA; signal-regulatory protein alpha; tyrosine-protein phosphatase non-receptor type substrate 1; mSIRP-alpha1; sirp-alpha-1; myD-1 antigen; SHP substrate 1; inhibitory receptor SHPS-1; signal-regulatory protein alpha-1; CD172 antigen-like family member A; brain immunological-like with tyrosine-based motifs; protein tyrosine phosphatase, non-receptor type substrate 1; brain Ig-like molecule with tyrosine-based activation motifs; Bit; P84; SIRP; SHP-1; CD172a; Ptpns1; SHPS-1; AI835480
• Gene ID: 19261
• mRNA Refseq: NM_001177646
• Protein Refseq: NP_001171117