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Recombinant Mouse SIRPA Protein, MIgG2a Fc-tagged, low endotoxin

Cat.No.: SIRPA-348M
Product Overview: Recombinant Mouse SIRPA Protein, MIgG2a Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Asn 373 (Accession # P97797-1(G365D)). Predicted N-terminus: Lys 32. This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 65.0 kDa. The protein migrates as 90-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source: HEK293
Species: Mouse
Tag : MIgG2a Fc
Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Bio-activity: Immobilized Recombinant Mouse SIRPA Protein, MIgG2a Fc-tagged, low endotoxin at 2 μg/mL (100 μL/well) can bind Mouse CD47, Fc Tag with a linear range of 8-63 ng/mL (QC tested).
Immobilized Human CD47 at 10 μg/mL (100 μL/well) can bind Recombinant Mouse SIRPA Protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 20-313 ng/mL (Routinely tested).
Molecular Mass: 65.0 kDa
Protein length: Lys 32 - Asn 373
Endotoxin: Less than 0.1 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Notes: Please avoid repeated freeze-thaw cycles.
Stability: No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name: Sirpa signal-regulatory protein alpha [ Mus musculus ]
Official Symbol: SIRPA
Synonyms: SIRPA; mSIRP-alpha1; sirp-alpha-1; myD-1 antigen; SHP substrate 1; Bit; P84; SIRP; SHP-1; CD172a; Ptpns1; SHPS-1; AI835480;
Gene ID: 19261
mRNA Refseq: NM_001177646
Protein Refseq: NP_001171117

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