|Product Overview:||Recombinant Mouse SIRPA Protein, MIgG2a Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Lys 32 - Asn 373 (Accession # P97797-1(G365D)). Predicted N-terminus: Lys 32. This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 65.0 kDa. The protein migrates as 90-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Tag :||MIgG2a Fc|
|Form:||Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity:||Immobilized Recombinant Mouse SIRPA Protein, MIgG2a Fc-tagged, low endotoxin at 2 μg/mL (100 μL/well) can bind Mouse CD47, Fc Tag with a linear range of 8-63 ng/mL (QC tested).
Immobilized Human CD47 at 10 μg/mL (100 μL/well) can bind Recombinant Mouse SIRPA Protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 20-313 ng/mL (Routinely tested).
|Molecular Mass:||65.0 kDa|
|Protein length:||Lys 32 - Asn 373|
|Endotoxin:||Less than 0.1 EU per μg by the LAL method.|
|Purity:||>95% as determined by SDS-PAGE.|
|Notes:||Please avoid repeated freeze-thaw cycles.|
|Stability:||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution:||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name:||Sirpa signal-regulatory protein alpha [ Mus musculus ]|
|Synonyms:||SIRPA; mSIRP-alpha1; sirp-alpha-1; myD-1 antigen; SHP substrate 1; Bit; P84; SIRP; SHP-1; CD172a; Ptpns1; SHPS-1; AI835480;|