Recombinant Mouse Sirt5 Protein, Myc/DDK-tagged

• Cat.No.: Sirt5-5884M
• Product Overview: Purified recombinant protein of mouse full-length sirtuin 5 (Sirt5), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as Uox.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 34.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Sirt5 sirtuin 5 [ Mus musculus (house mouse) ]
• Official Symbol: Sirt5
• Synonyms: SIRT5; sirtuin 5 (silent mating type information regulation 2 homolog) 5 (S. cerevisiae); NAD-dependent lysine demalonylase and desuccinylase sirtuin-5, mitochondrial; SIR2-like protein 5; NAD-dependent deacetylase sirtuin-5; AV001953; 0610012J09Rik; 1500032M05Rik
• Gene ID: 68346
• mRNA Refseq: NM_178848
• Protein Refseq: NP_849179
• UniProt ID: Q8K2C6