Recombinant Mouse Smad2 Protein, Myc/DDK-tagged

• Cat.No.: Smad2-5950M
• Product Overview: Purified recombinant protein of mouse full-length SMAD family member 2 (Smad2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 52.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Smad2 SMAD family member 2 [ Mus musculus (house mouse) ]
• Official Symbol: Smad2
• Synonyms: SMAD2; MAD homolog 2 (Drosophila); mothers against decapentaplegic homolog 2; Smad 2; SMAD family member 2; mad-related protein 2; mothers against DPP homolog 2; Madh2; Madr2; mMad2; Smad-2; 7120426M23Rik
• Gene ID: 17126
• mRNA Refseq: NM_010754
• Protein Refseq: NP_034884
• UniProt ID: Q62432