Recombinant Mouse Smpd3 Protein, Myc/DDK-tagged

• Cat.No.: Smpd3-5977M
• Product Overview: Purified recombinant protein of mouse full-length sphingomyelin phosphodiesterase 3, neutral (Smpd3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization. Binds to anionic phospholipids (APLs) such as phosphatidylserine (PS) and phosphatidic acid (PA) that modulate enzymatic activity and subcellular location. May be involved in IL-1-beta-induced JNK activation in hepatocytes. May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF-kappa-B activation under inflammatory conditions.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 71.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Smpd3 sphingomyelin phosphodiesterase 3, neutral [ Mus musculus (house mouse) ]
• Official Symbol: Smpd3
• Synonyms: SMPD3; sphingomyelin phosphodiesterase 3, neutral; sphingomyelin phosphodiesterase 3; nSMase-2; neutral sphingomyelinase 2; neutral sphingomyelinase II; fro; nSMase2; AI427456; AW537966; 4631433G07Rik
• Gene ID: 58994
• mRNA Refseq: NM_021491
• Protein Refseq: NP_067466
• UniProt ID: Q9JJY3