Recombinant Mouse Snapin Protein, Myc/DDK-tagged

• Cat.No.: Snapin-5995M
• Product Overview: Purified recombinant protein of mouse full-length SNAP-associated protein (cDNA clone MGC:12076 IMAGE:3708620), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells. As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor.
• Molecular Mass: 9.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Snapin SNAP-associated protein [ Mus musculus (house mouse) ]
• Official Symbol: Snapin
• Synonyms: Snapin; SNAP-associated protein; Sna; Bloc; 25kDa; Snapap; Bloc1s7; AA407989; AV026596; Snap25bp; SNARE-associated protein Snapin; BLOC-1 subunit 7; biogenesis of lysosome-related organelles complex 1 subunit 7; synaptosomal-associated protein 25 binding protein; synaptosomal-associated protein, 25 kDa, binding protein
• Gene ID: 20615
• mRNA Refseq: NM_133854
• Protein Refseq: NP_598615
• UniProt ID: Q9Z266