| Description : |
Spr also known as Sepiapterin reductase, belongs to the Short-chain dehydrogenase/reductase (Sdr) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. Spr is an essential enzyme for the biosynthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydrolases including tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Defects in Spr cause DOPA-responsive dystonia defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. |
| Source : |
E. coli |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Liquid. In 20mM Tris-HCl buffer (pH8.5) containing 1mM DTT, 10% glycerol |
| Molecular Mass : |
30.3kDa (285aa), confirmed by MALDI-TOF |
| Protein length : |
1-262aa |
| AA Sequence : |
MGSSHHHHHH SSGLVPRGSH MGSMEAGGLG CAVCVLTGAS RGFGRALAPQ LARLLSPGSV MLVSARSESM LRQLKEELGA QQPDLKVVLAAADLGTEAGV QRLLSAVREL PRPEGLQRLL LINNAATLGD VSKGFLNVND LAEVNNYWAL NLTSMLCLTS GTLNAFQDSP GLSKTVVNISSLCALQPYKG WGLYCAGKAA RDMLYQVLAA EEPSVRVLSY APGPLDNDMQ QLARETSKDP ELRSKLQKLK SDGALVDCGT SAQKLLGLLQKDTFQSGAHV DFYDC |
| Purity : |
> 95% by SDS - PAGE |
| Applications : |
SDS-PAGE |
| Storage : |
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : |
1mg/ml (determined by Absorbance at 280nm) |
