Recombinant Mouse Srpk2 Protein, Myc/DDK-tagged

• Cat.No.: Srpk2-6131M
• Product Overview: Purified recombinant protein of mouse full-length serine/arginine-rich protein specific kinase 2 (Srpk2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Promotes neuronal apoptosis by up-regulating cyclin-D1 (CCND1) expression. This is done by the phosphorylation of SRSF2, leading to the suppression of p53/TP53 phosphorylation thereby relieving the repressive effect of p53/TP53 on cyclin-D1 (CCND1) expression. Phosphorylates ACIN1, and redistributes it from the nuclear speckles to the nucleoplasm, resulting in cyclin A1 but not cyclin A2 up-regulation. Plays an essential role in spliceosomal B complex formation via the phosphorylation of DDX23/PRP28. Probably by phosphorylating DDX23, leads to the suppression of incorrect R-loops formed during transcription; R-loops are composed of a DNA:RNA hybrid and the associated non-template single-stranded DNA.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 76.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Srpk2 serine/arginine-rich protein specific kinase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Srpk2
• Synonyms: SRPK2; serine/arginine-rich protein specific kinase 2; SRSF protein kinase 2; SFRS protein kinase 2; WW domain binding protein 6; SR-protein-specific kinase 2; serine/threonine-protein kinase SRPK2; serine/arginine-rich protein-specific kinase 2; Wbp6; mSRPK2; AW226533; AW492537; AW547358
• Gene ID: 20817
• mRNA Refseq: NM_009274
• Protein Refseq: NP_033300
• UniProt ID: O54781