Recombinant Mouse TDG Protein, His-tagged

• Cat.No.: TDG-338M
• Product Overview: Full length Recombinant Mouse TDG (Thymine-DNA Glycosylase) Protein is produced in SF9 Insect Cells and is fused with a His tag at the N-terminal.

Specification

• Description: TDG (UniProt/Swiss-Prot entry Q13569) belongs to the TDG/mug DNA glycosylase family. It removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. It is also able to remove thymine from C/T and T/T mispairings, although with lower activity. Furrther, TDG plays a key role in active DNA demethylation as it binds to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), but not 5-hydroxymethylcytosine (5hmC), and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine.
• Source: Insect Cells
• Species: Mouse
• Tag: Flag
• Form: 20mM Tris-Cl pH 7.6, 1 mM EDTA, 0.15 M NaCl, 10% glycerol, 0.5 mM PMSF and 1 mM DTT.
• Purity: Purified using FPLC, >95% purity as determined by SDS-PAGE.
• Notes: This product is for research use only. Not for use in diagnostic or therapeutic procedures.
• Stability: Guaranteed stable for 2 years from date of receipt when stored properly.
• Storage: Store at -80 centigrade.
• Concentration: 0.5 µg/µl
• Synonyms: HTDG

Gene Information

• Synonyms: HTDG