| Cat.No. : |
TDG-338M |
| Product Overview : |
Full length Recombinant Mouse TDG (Thymine-DNA Glycosylase) Protein is produced in SF9 Insect Cells and is fused with a His tag at the N-terminal. |
| Description : |
TDG (UniProt/Swiss-Prot entry Q13569) belongs to the TDG/mug DNA glycosylase family. It removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. It is also able to remove thymine from C/T and T/T mispairings, although with lower activity. Furrther, TDG plays a key role in active DNA demethylation as it binds to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), but not 5-hydroxymethylcytosine (5hmC), and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. |
| Source : |
Insect Cells |
| Species : |
Mouse |
| Tag : |
Flag |
| Form : |
20mM Tris-Cl pH 7.6, 1 mM EDTA, 0.15 M NaCl, 10% glycerol, 0.5 mM PMSF and 1 mM DTT. |
| Purity : |
Purified using FPLC, >95% purity as determined by SDS-PAGE. |
| Notes : |
This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Stability : |
Guaranteed stable for 2 years from date of receipt when stored properly. |
| Storage : |
Store at -80 centigrade. |
| Concentration : |
0.5 µg/µl |
| Synonyms : |
HTDG |
