Recombinant Mouse TDG Protein, His-tagged


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Recombinant Mouse TDG Protein, His-tagged

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Cat.No. : TDG-338M
Product Overview : Full length Recombinant Mouse TDG (Thymine-DNA Glycosylase) Protein is produced in SF9 Insect Cells and is fused with a His tag at the N-terminal.
Description : TDG (UniProt/Swiss-Prot entry Q13569) belongs to the TDG/mug DNA glycosylase family. It removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. It is also able to remove thymine from C/T and T/T mispairings, although with lower activity. Furrther, TDG plays a key role in active DNA demethylation as it binds to 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), but not 5-hydroxymethylcytosine (5hmC), and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine.
Source : Insect Cells
Species : Mouse
Tag : Flag
Form : 20mM Tris-Cl pH 7.6, 1 mM EDTA, 0.15 M NaCl, 10% glycerol, 0.5 mM PMSF and 1 mM DTT.
Purity : Purified using FPLC, >95% purity as determined by SDS-PAGE.
Notes : This product is for research use only. Not for use in diagnostic or therapeutic procedures.
Stability : Guaranteed stable for 2 years from date of receipt when stored properly.
Storage : Store at -80 centigrade.
Concentration : 0.5 µg/µl
Synonyms : HTDG

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