Active Recombinant Mouse TNFSF14 protein

• Cat.No.: TNFSF14-2020M
• Product Overview: Active Recombinant Mouse TNFSF14 protein(72-239 aa) was expressed in Escherichia coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Protein Length: 72-239 aa
• Form: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4.
• Bio-activity: Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using human HT-29 cells is less than 2 μg/ml, corresponding to a specific activity of > 500 IU/mg in the presence of murine anti-polyHistidine monoclonal antibody and rHuIFN-γ.
• Molecular Mass: Approximately 18.4 kDa, a single non-glycosylated polypeptide chain containing 168 amino acids.
• AASequence: DGGKGSWEKL IQDQRSHQAN PAAHLTGANA SLIGIGGPLL WETRLGLAFL RGLTYHDGAL VTMEPGYYYV YSKVQLSGVG CPQGLANGLP ITHGLYKRTS RYPKELELLV SRRSPCGRAN SSRVWWDSSF LGGVVHLEAG EEVVVRVPGN RLVRPRDGTR SYFGAFMV
• Endotoxin: Less than 1 EU/µg of rMuLIGHT/TNFSF14 as determined by LAL method.
• Purity: > 96 % by SDS-PAGE and HPLC analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 100 mM HAc to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C.

Gene Information

• Gene Name: Tnfsf14 tumor necrosis factor (ligand) superfamily, member 14 [ Mus musculus ]
• Official Symbol: TNFSF14
• Synonyms: TNFSF14; tumor necrosis factor (ligand) superfamily, member 14; tumor necrosis factor ligand superfamily member 14; LTg; HVEML; LIGHT; Ly113; HVEM-L
• Gene ID: 50930
• mRNA Refseq: NM_019418
• Protein Refseq: NP_062291