Recombinant Mouse TrkB protein(Met1-His429), His-tagged

• Cat.No.: Ntrk2-1353M
• Product Overview: Recombinant Mouse NTRK2 (P15209-2) (Met 1-His 429) was expressed in HEK293, with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-His429
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant Mouse/Human BDNF Protein (Native) at 10 μg/ml (100 μl/well) can bind biotinylated mouse TrkB-His with a linear range of 10-80 ng/ml.
• Molecular Mass: The secreted recombinant mouse NTRK2 consists of 409amino acids and has a calculated molecular mass of 46 kDa. The apparent molecular mass of rm NTRK2 is approximately 75-85 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.