Recombinant Mouse Trmt6 Protein, Myc/DDK-tagged

• Cat.No.: Trmt6-6668M
• Product Overview: Purified recombinant protein of mouse full-length tRNA methyltransferase 6 (Trmt6), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Substrate-binding subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA. Together with the TRMT61A catalytic subunit, part of a mRNA N1-methyltransferase complex that mediates methylation of adenosine residues at the N1 position of a small subset of mRNAs: N1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 55.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Trmt6 tRNA methyltransferase 6 [ Mus musculus (house mouse) ]
• Official Symbol: Trmt6
• Synonyms: TRMT6; tRNA methyltransferase 6 homolog (S. cerevisiae); tRNA (adenine(58)-N(1))-methyltransferase non-catalytic subunit TRM6; tRNA(m1A58)MTase subunit TRM6; tRNA(m1A58)-methyltransferase subunit TRM6; tRNA (adenine-N(1)-)-methyltransferase non-catalytic subunit TRM6; CGI-09; AU016412; AW492787; mKIAA1153; RP23-77H16.2; 3300001M20Rik
• Gene ID: 66926
• mRNA Refseq: NM_175113
• Protein Refseq: NP_780322
• UniProt ID: Q8CE96