Recombinant Mouse Uba3 Protein, Myc/DDK-tagged

• Cat.No.: Uba3-6766M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-like modifier activating enzyme 3 (Uba3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: The protein encoded by this gene is the catalytic subunit of the enzyme that activates NEDD8, a ubiquitin-like molecule that binds to its target proteins through an enzymatic reaction analagous to ubiquitylation. Embryonic mice deficient for this protein die prior to implantation and display apoptosis of the inner cell mass. Trophoblastic cells cannot enter S phase, demonstrating that this gene is required for cell cycle progression during embryogenesis. Two pseudogenes have been found for this gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2014]
• Molecular Mass: 49.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Uba3 ubiquitin-like modifier activating enzyme 3 [ Mus musculus (house mouse) ]
• Official Symbol: Uba3
• Synonyms: UBA3; ubiquitin-like modifier activating enzyme 3; NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; ubiquitin activating enzyme 3; ubiquitin-activating enzyme 3; ubiquitin-activating enzyme E1C; ubiquitin-like modifier-activating enzyme 3; Ube1c; AI256736; AI848246; AW546539; A830034N06Rik
• Gene ID: 22200
• mRNA Refseq: NM_011666
• Protein Refseq: NP_035796
• UniProt ID: Q8C878