Recombinant Mouse Ube2f Protein, Myc/DDK-tagged

• Cat.No.: Ube2f-6786M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-conjugating enzyme E2F (putative) (Ube2f), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 21.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ube2f ubiquitin-conjugating enzyme E2F (putative) [ Mus musculus (house mouse) ]
• Official Symbol: Ube2f
• Synonyms: UBE2F; ubiquitin-conjugating enzyme E2F (putative); NEDD8-conjugating enzyme UBE2F; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; NEDD8 carrier protein UBE2F; ubiquitin-conjugating enzyme E2 F; AI851109; 2510010F15Rik
• Gene ID: 67921
• mRNA Refseq: NM_026454
• Protein Refseq: NP_080730
• UniProt ID: Q9CY34