Recombinant Mouse Ube2i Protein, Myc/DDK-tagged

• Cat.No.: Ube2i-6789M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-conjugating enzyme E2I (cDNA clone MGC:46871 IMAGE:4951933), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Accepts the ubiquitin-like proteins SUMO1, SUMO2 and SUMO3 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2, CBX4 and ZNF451. Can catalyze the formation of poly-SUMO chains. Essential for nuclear architecture, chromosome segregation and embryonic viability. Necessary for sumoylation of FOXL2 and KAT5. Sumoylates p53/TP53 at 'Lys-386'. Mediates sumoylation of ERCC6 which is essential for its transcription-coupled nucleotide excision repair activity.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 11.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ube2i ubiquitin-conjugating enzyme E2I [ Mus musculus (house mouse) ]
• Official Symbol: Ube2i
• Synonyms: Ube2i; ubiquitin-conjugating enzyme E2I; UBC9; Ubce9; Ubce2i; 5830467E05Rik; SUMO-conjugating enzyme UBC9; RING-type E3 SUMO transferase UBC9; SUMO-protein ligase; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin-protein ligase I; EC 6.3.2.19
• Gene ID: 22196
• mRNA Refseq: NM_011665
• Protein Refseq: NP_035795
• UniProt ID: P63280