Recombinant Mouse Ube2s Protein, Myc/DDK-tagged

• Cat.No.: Ube2s-6798M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-conjugating enzyme E2S (Ube2s), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 24.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ube2s ubiquitin-conjugating enzyme E2S [ Mus musculus (house mouse) ]
• Official Symbol: Ube2s
• Synonyms: UBE2S; ubiquitin-conjugating enzyme E2S; ubiquitin-conjugating enzyme E2 S; E2-EPF5; ubiquitin-protein ligase S; ubiquitin carrier protein S; ubiquitin-conjugating enzyme E2-EPF5; ubiquitin-conjugating enzyme E2-24 kDa; E2-EPF; AA409170; 0910001J09Rik; 6720465F12Rik; MGC101982
• Gene ID: 77891
• mRNA Refseq: NM_133777
• Protein Refseq: NP_598538
• UniProt ID: Q921J4