Recombinant Mouse Usp16 Protein, Myc/DDK-tagged

• Cat.No.: Usp16-6867M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin specific peptidase 16 (Usp16), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Specifically deubiquitinates 'Lys-120' of histone H2A (H2AK119Ub), a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-11' of histone H3 (H3S10ph), and is required for chromosome segregation when cells enter into mitosis. In resting B- and T-lymphocytes, phosphorylation by AURKB leads to enhance its activity, thereby maintaining transcription in resting lymphocytes. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B.
• Source: HEK293T
• Species: Mouse
• Tag: Myc&DDK
• Molecular Mass: 93.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Usp16 ubiquitin specific peptidase 16 [ Mus musculus (house mouse) ]
• Official Symbol: Usp16
• Synonyms: Usp16; ubiquitin specific peptidase 16; UBP-; UBPM; 1200004E02Rik; 2810483I07Rik; 6330514E22Rik; ubiquitin carboxyl-terminal hydrolase 16; deubiquitinating enzyme 16; ubiquitin specific protease 16; ubiquitin thioesterase 16; ubiquitin thiolesterase 16; ubiquitin-specific-processing protease 16; EC 3.4.19.12
• Gene ID: 74112
• mRNA Refseq: NM_024258
• Protein Refseq: NP_077220
• UniProt ID: Q99LG0