Active Recombinant Mouse Usp18 Protein, His-tagged

• Cat.No.: Usp18-32M
• Product Overview: Recombinant mouse Usp18 protein (Ser47 - Ser368) contains an N-terminal 6-His tag was expressed in Spodoptera frugiperda, Sf 21 (baculovirus).

Specification

• Description: Involved in the regulation of inflammatory response to interferon type 1. Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.
• Source: Insect cell
• Species: Human
• Tag: His
• Bio-activity: Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant murine USP18 concentration of 100-250 nM when using ISG15-AMC as a substrate.
• Molecular Mass: 38 kDa
• Purity: >98%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  24 months from date of receipt, -70 centigrade as supplied.
  3 months, -70 centigrade under sterile conditions after opening.
• Storage Buffer: X mg/mL (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% (v/v) Glycerol, 5 mM DTT and 1 mM EDTA

Gene Information

• Gene Name: Usp18 ubiquitin specific peptidase 18 [ Mus musculus (house mouse) ]
• Official Symbol: Usp18
• Synonyms: 43 kDa ISG15-specific protease; ISG15-specific-processing protease; ubiquitin specific protease 15; ubiquitin specific protease 18; ubl thioesterase 18; ubl thiolesterase 18; EC 3.1.2.-; Usp18; ubiquitin specific peptidase 18; UBP43; Ubp15; AW047653; 1110058H21Rik
• Gene ID: 24110
• mRNA Refseq: NM_011909
• Protein Refseq: NP_036039
• UniProt ID: Q9WTV6