Recombinant Mouse Usp37 Protein, Myc/DDK-tagged

• Cat.No.: Usp37-6876M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin specific peptidase 37 (Usp37), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2). Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 110 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Usp37 ubiquitin specific peptidase 37 [ Mus musculus (house mouse) ]
• Official Symbol: Usp37
• Synonyms: USP37; ubiquitin specific peptidase 37; ubiquitin carboxyl-terminal hydrolase 37; ubiquitin thioesterase 37; deubiquitinating enzyme 37; ubiquitin thiolesterase 37; ubiquitin specific protease 37; ubiquitin-specific-processing protease 37; 4932415L06Rik; C330008N13Rik
• Gene ID: 319651
• mRNA Refseq: NM_176972
• Protein Refseq: NP_795946
• UniProt ID: Q8C0R0